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KMID : 0613820090190111598
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Journal of Life Science
2009 Volume.19 No. 11 p.1598 ~ p.1604
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Effects of Onion Flesh and Peel on Chemical Components, Antioxidant and Anticancer Activities
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Jang Joo-Ri
Lim Sun-Young
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Abstract
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In order to determine chemical components of onion flesh and peel, general nutrients, vitamin C, and total flavonoids were measured. Onion peel showed less moisture (14.3%) and no vitamin C compared to onion flesh. Onion peel contained more amounts of total flavonoids compared to onion flesh. In addition, the inhibitory effects of solvent extracts from onion flesh and peel on H₂O₂-induced oxidative stress and growth of cancer cell lines (AGS human gastric adenocarcinoma and HT-29 human colon cancer cells) were investigated. Acetone with methylene chloride (A+M) and methanol (MeOH) extracts from onion flesh and peel appeared to significantly reduce the levels of intracellular reactive oxygen species (ROS) (p＜0.05) and a greater antioxidant effect was observed in onion peel. Among fractions, 85% aq. methanol showed a higher protective activity against oxidative stress in both flesh and peel and there was no effect in the water and hexane fractions. The growth of cancer cells exposed to medium containing extracts and fractions from onion flesh and peel was inhibited dose-dependently. The growth of AGS was inhibited more in both flesh and peel compared to HT-29, and onion peel was more effective than onion flesh. Among fractions, 85% aq. methanol showed the greatest effect on growth inhibition in both flesh and peel. IC?? values of 85% aq. methanol fraction from onion flesh and peel on AGS were 0.04 and 0.03 ㎎/㎖, respectively, while those on HT-29 were 0.23 and 0.04 ㎎/㎖. From our results, 85% aq. methanol fraction had an inhibitory effect against oxidative stress and growth of cancer cells, suggesting that it may contain biological active compounds.
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KEYWORD
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Onion flesh, onion peel, antioxidant, human cancer cell, growth inhibition
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